Co-localization and correlation of Alz50 and Tsg101 immunoreactivity in P301S mouse brain. a, b. Laser-scanning confocal microscopic imaging of Tsg101 and Alz50 staining in the CA1 (a) and CA3 (b) hippocampal region. c, d. Scattered plot for the Pearson’s correlation analysis of Alz50 and Tsg101 signal for CA1 (c) and CA3 regions (d), respectively. Each dot represents an individual animal from P301S + VEH group (red) and P301S + GSK1482160 group (blue). e. Schematic diagram of PLA, only occurs when proteins are in close proximity (20–50 nm). f. Representative laser-scanning confocal microscopic images of PLA signals between Tsg101 and Alz50 (red) with Dapi (blue) at CA1 and CA3 of the hippocampus. g. Quantification of the PLA+ immunopuncta for Tsg101-Alz50 at CA1 and CA3 hippocampal regions. ***p <0.001, ****p < 0.0001 compared with WT + VEH group; #p < 0.05 and ##p < 0.01, compared with P301S + VEH group; n.s denotes no significance as determined by one-way ANOVA (alpha = 0.05) and Dunnett’s post-hoc. n = (4, 4, 4) mice for WT + VEH, P301S + VEH and P301S + GSK groups. Graphs indicate mean ± s.e.m.