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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Modeling the β-secretase cleavage site and humanizing amyloid-beta precursor protein in rat and mouse to study Alzheimer’s disease

Fig. 2

Humanization of the Aβ sequence in mouse affects APP processing. a Western blot analysis of APP protein in the cerebrum of 14 weeks old WT and Apphu/hu mice (n = 6). B63 antibody detects full length APP (APP FL) and C-terminal fragments (CTF, longer exposure). 82E1 antibody specifically detects human Aβ1-22, and hence the human B-CTF, independently confirming that the mouse App gene was successfully humanized. β- ACTIN was used as loading control. b Quantification of APP protein in relative intensities (n = 6, mean ± SD, *** p < 0.0001 two tailed t-test). c ELISA analysis of soluble Aβ expressed as pg/g tissue. BD, below detection limit; ND, not determined (n = 6, mean ± SD, ***p < 0.0001 two tailed t-test). d Immunoblot of total MAPT in mouse cerebrum using the 3Rtau-specific antibody RD3, 4Rtau-specific antibody RD4 and an antibody against total tau. The MAPT ladder shows recombinant human MAPT (0N3R, 0N4R, 1N3R, 1N4R, 2N3R, 2N4R). Notice that mouse MAPT proteins migrate faster than the corresponding human splice variants. β- ACTIN was used as the loading control. e Quantification of MAPT relative to WT samples ((n = 6, mean ± SD)

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