Fig. 11From: CNS axonal degeneration and transport deficits at the optic nerve head precede structural and functional loss of retinal ganglion cells in a mouse model of glaucomaPartial axonal transport deficits at the ONH is associated with early stages of optic nerve degeneration in Dex-treated mice. Three months old C57BL/6 J mice were injected unilaterally with Veh and Dex once a week for 5 weeks and axonal anterograde transport was tracked along the entire length of optic nerve using fluorescently tagged CTB. CTB Alexa Fluor 555 (Red) and CTB Alexa Fluor 488 (Green) were used to tract transport deficits in Veh and Dex-injected eyes respectively n = 3. a Graphical representation of serial cross sections of optic nerve at the proximal (close to glial lamina), center and distal (close to optic chiasma) regions. b Serial cross sections of optic nerve images show partial accumulation of CTB (green fluorescence) at proximal ON region in 5 weeks Dex injected eyes compared to contralateral Veh-injected eyes (CTB-red fluorescence). c Fluorescent intensities of CTB from proximal to distal ON in 5 weeks of Veh and Dex injected mice was analyzed by Image J and shown graphically. For each optic nerve, an average of fluorescent intensities of 3 images from each region were taken into the consideration. The data are shown as mean ± SD (n = 3, One WAY ANOVA with multiple comparison; ns = not significant, *p = 0.02)Back to article page