Skip to main content
Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: CNS axonal degeneration and transport deficits at the optic nerve head precede structural and functional loss of retinal ganglion cells in a mouse model of glaucoma

Fig. 3

Dex-induced OHT leads to optic nerve axonal degeneration in mice. Optic nerves from 10-week Veh or Dex-injected mice were subjected to PPD staining (a & b) and TEM imaging (C) to examine optic nerve degeneration. a Representative images of PPD stained optic nerves show severe axonal degeneration along with gliosis and glial scar in 10 weeks Dex-injected mice compared to Veh-injected mice. b The mean axon counts in 10 weeks Dex-injected mice show a significant reduction in number of healthy axons compared to Veh-injected mice. Data are shown as mean ± SD (n = 5 in Veh and n = 4 in Dex, unpaired t-test, two-tailed, ***p = 0.0004). c TEM images confirm extensive glial scar and degenerated axons in Dex-injected mice compared to Veh-injected mice. In addition, we observed unmyelinated axons, vacuoles, infiltrating immune cells that were associated with optic nerve degeneration in Dex-injected mice compared to Veh-injected mice (n = 4). (* unmyelinated axons; # glial scar; ‡ vacuoles; Mφ infiltrating immune cells)

Back to article page