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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: CNS axonal degeneration and transport deficits at the optic nerve head precede structural and functional loss of retinal ganglion cells in a mouse model of glaucoma

Fig. 4

Dex-induced glaucomatous neurodegeneration is IOP dependent. Three-month old C57BL/6 J mice were injected bilaterally once a week for 8 weeks with either Veh or Dex, and given topical ocular eye drops of either control (water) or Cosopt (b.i.d) + Latanoprost (once a day) for 8 weeks. a Dex injected mice receiving control eye drops (DexControl) show sustained and significant IOP elevation compared to Veh injected mice receiving control eye drops (VehControl), IOP is significantly reduced in Dex-injected mice receiving Cosopt+Latanoprost eye drops (DexCosopt + Latanoprost) compared to DexControl mice. Data are shown as mean ± SD (n = 8 to 10 in each group, 2-WAY ANOVA with multiple comparison, #p < 0.0001). b Reduction of IOP prevents Dex-induced RGC functional loss. PERG was measured in mice treated with periocular injections of Veh or Dex along with or without topical ocular eye drops of Cosopt+Latanoprost. DexControl mice demonstrate significant reduction in PERG amplitudes compared to VehControl or DexCosopt + Latanoprost mice. There is no difference in PERG amplitudes between VehControl and DexCosopt + Latanoprost mice. Data are shown as mean ± SD (n = 8 to 10 in each group, One WAY ANOVA with multiple comparison). c Reduction of IOP prevents Dex-induced RGC structural loss. Representative images of whole mount retina immunostained with RBPMS and corresponding RGC counts are shown. There is a significant reduction in RGC numbers in DexControl mice compared to VehControl mice, whereas DexCosopt + Latanoprost mice did not show RGC loss compared to VehControl mice. Data are shown as mean ± SD (n = 4 or 5, One WAY ANOVA with multiple comparison, ***p < 0.0001). d Reduction of IOP prevents Dex-induced optic nerve degeneration. PPD stained optic nerve axons were counted and total number of axons per optic nerve are represented in a dot plot. DexControl mice showed significant axonal degeneration compared to VehControl mice, and axonal degeneration was prevented in DexCosopt + Latanoprost mice. Data are shown as mean ± SD (n = 4, One WAY ANOVA with multiple comparison, *p = 0.01)

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