Table 1 List of antibodies used in the study
Primary Antibody | Source | Catalog number | Dilutions factor |
|---|---|---|---|
Fibronectin (FN) | Santa Cruz Biotechnology | sc-18,825 | 1 in 200 |
Collagen1 (ColI) | Novus Biology | NB600–408 | 1 in 200 |
Phalloidin 650 | Cell Signaling | 12956S | 1 in 50 |
Laminin | Sigma | L9393 | 1 in 200 |
αSMA | Abcam | ab7817 | 1 in 200 |
GFAP | Abcam | ab10062 | 1 in 100 |
NF-H | Abcam | ab8135 | 1 in 100 |
F4/80 | Abcam | ab6640 | 1 in 200 |
TNF-alpha | Abcam | ab1793 | 1 in 100 |
CoxIV | Abcam | ab16056 | 1 in 100 |
RBPMS | Gentex | GTX118619 | 1 in 200 |
Cholera Toxin Subunit B (Recombinant), Alexa Fluor™ 555 Conjugate | Thermo Fisher Scientific | C34776 | 3 μl of 0.1% CTB/eye |
Cholera Toxin Subunit B (Recombinant), Alexa Fluor™ 488 Conjugate | Thermo Fisher Scientific | C34775 | 3 μl of 0.1% CTB/eye |
Secondary Antibody | Source | Catalog number | Dilutions factor |
Goat anti rabbit 568 | Invitrogen | A11011 | 1 in 500 |
Goat anti rabbit 488 | Invitrogen | A11034 | 1 in 500 |
Goat anti rabbit 680 | Life technologies | A21076 | 1 in 500 |
Goat anti mouse 568 | Life technologies | A11031 | 1 in 500 |
Goat anti mouse 488 | Invitrogen | A11001 | 1 in 500 |