Fig. 1
From: Peroxiredoxin 6 mediates protective function of astrocytes in Aβ proteostasis
Development and characterization of APP/Prdx6Tg and APP/Prdx6+/− lines. a Breeding scheme: hemizygous APPSWE/PS1dE9 AD Tg mice (APP/PS11/0;Prdx6+/+) were mated to mice, which on the background of endogenous Prdx6 transgenically over-express wild type Prdx6129X1/SvJ allele (Prdx6+/+/Tg1/1), wild type C57BL/6 J mice (Prdx6+/+) or Prdx6 knock out mice (Prdx6−/−). Female F1 progeny of the following genotypes APP/PS11/0;Prdx6+/+Tg1/0 (APP/Prdx6Tg), APP/PS11/0;Prdx6+/+ (APP/Prdx6+/+), and APP/PS11/0;Prdx6+/− (APP/Prdx6+/−) were analyzed in this study. b, c Shown is Prdx6 genotyping in parental and F1 generations, respectively. Wild type Prdx6 PCR yields a single 199 bp band, whose variable intensity can side-to-side distinguish endogenous allele from transgenic overexpression, while 315 bp PCR product indicates transgenically disrupted Prdx6 allele. Altered Prdx6 expression does not affect mRNA and protein level for APP and GFAP as evidenced by qRT-PCR analysis in d and quantitative Western blotting in e and f in mice aged 4–6 weeks, respectively. Values in d and e express a fold change relative to APP/Prdx6+/+ line and represent mean (+SEM) from n = 6–8 mice per genotype in d and n = 5–7 mice per genotype in f. Altered Prdx6 expression does not affect load of astrocytes before the onset of Aβ deposition. g Representative microphotographs of coronal cross-sections through the dorsal hippocampus from 3-month-old female mice of indicated genotypes immunolabeled against GFAP. h Quantification of GFAP+ astrocyte load in the dentate hilus. Values represent mean (+SEM) from n = 5 mice per genotype. i ELISA analysis of soluble (DEA-extractable) Aβx-40 and Aβx-42 levels in the brain cortex of mice aged 2 months. Values represent mean (+SEM) from n = 7–10 animals per genotype. p = 0.0008 for Prdx6 mRNA in d and p < 0.0001 for PRDX6 protein in f (ANOVA); *p < 0.05, ***p < 0.001, and ****p < 0.0001 (Holm-Sidak’s post-hoc test). ANOVA for APP and Gfap mRNA in d, APP and GFAP protein in f, GFAP+ astrocyte load in h, and Aβx-40 and Aβx-42 levels in i is not significant. Abbreviations: gl - granular layer, h - hilus. Scale bar: 150 μm in g