Fig. 2
From: Peroxiredoxin 6 mediates protective function of astrocytes in Aβ proteostasis
Aβ load in APPSWE/PS1dE9 mice varies inversely with Prdx6 gene expression level. a and c Representative microphotographs of coronal cross-sections through the somatosensory cortex and the dorsal hippocampus from 10-month-old female mice of indicated genotypes, which were stained for fibrillar Aβ plaques with Thioflavin-S or immunolabeled with HJ3.4 clone directed against the N-terminus of Aβ peptide, respectively. Quantitative analysis of fibrillar b and immunopositive d Aβ plaque load in the brain cortex and in the hippocampus evidencing suppression of Aβ deposition in Prdx6 overexpressing mice and increased Aβ deposition in Prdx6 haplodeficient mice. Values represent mean (+SEM) from n = 9–12 female mice per genotype. e and f ELISA analysis of soluble (DEA-extractable) and total (FA-extractable) Aβx-40 and Aβx-42 levels in the brain cortex of female 10-month-old mice, respectively. Values represent mean (+SEM) from n = 7–13 animals per genotype. p < 0.0001 in b, d, e, and f (ANOVA); *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 (Holm-Sidak’s post-hoc test). Abbreviations: CA1 – cornu Ammonis sector 1, Crtx – cortex, DG – dentate gyrus, Hip – hippocampus. Scale bars: 750 μm in a and 50 μm in c