Fig. 8

Prdx6 expression modulates plaque seeding. Shown are representative images of nascent plaques in female mice aged 6 months of indicated genotypes, which were co-labeled with X-34 (fibrillar core), and anti-Aβ (HJ3.4 clone) and anti-GFAP antibodies in a and with X-34, anti-Aβ (HJ3.4 clone), and anti-Iba1 antibodies in b evidencing an inverse relationship between Prdx6 expression level and the numerical density of nascent plaques and the numerical density of nascent plaques devoid of GFAP⁺ and Iba1⁺ cells. Quantitative analysis of nascent plaque numerical density in the brain cortex performed on X-34/Aβ/GFAP co-labeled sections in c, and on X-34/Aβ/Iba1 co-labeled sections in d. In this analysis nascent plaques were arbitrary defined as having no X-34⁺ core or having X-34⁺ core < 40 μm². Black bars represent number of plaques devoid of GFAP⁺ or Iba1⁺ cells /mm² and are superimposed on grey bars representing the total number of plaques /mm² for a given genotype. Values in c and d represent mean (+SEM) from n = 6 female mice per genotype. p < 0.0001 in c and d (ANOVA); ^●●p < 0.01, ^●●●p < 0.001, and ^●●●●p < 0.0001 denote significance for the density of GFAP and Iba1 devoid plaques across genotypes; *p < 0.05, and ***p < 0.001, denote significance for the density of all plaques (Holm-Sidak’s post-hoc test). Scale bar: 20 μm in a and b