Fig. 7

SKT82 and DMR7 inhibit tau pathology in vivo. a IHC staining of hyperphosphorylated tau with the AT8 antibody reveals abundant tau pathology in AD-tau injected 5xFAD mice 3 months post-injection compared to PBS-injected mice at low magnification; scale bar = 300 μm. b High magnification of hyperphosphorylated, AT8-positive tau pathology in the ipsilateral and contralateral dentate gyrus and CA3 region of the hippocampus demonstrating neuritic plaque tau pathology; scale bar = 50 μm. c Quantification of AT8-positive area reveals treatment with SKT82 significantly reduced the amount of AT8-positive tau pathology on the ipsilateral side, and DMR7 and SKT82 show a strong reduction of tau pathology on the contralateral side approaching statistical signfigance. Each mAb treatment group was individually compared to the corresponding IgG isotype control group by unpaired t-test n = 9–13 mice/group, each point represents mean % positive AT8 area for 3–5 sections per mouse. d Sequential extraction of soluble and sarkosyl-insoluble tau fractions from both ipsilateral and contralateral mouse hippocampi were evaluated for levels of hyperphosphorylated tau (PHF1) and total tau by immunoblot