Skip to main content
Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Trem2 Y38C mutation and loss of Trem2 impairs neuronal synapses in adult mice

Fig. 4

Decreased synaptic elements in adult Trem2Y38C/Y38C and Trem2−/− mice. Microdissected a,b cortices and c,d hippocampi of WT, Trem2Y38C/Y38C and Trem2−/− mice were analyzed using western blot with antibodies against PSD95 and synaptophysin. a,b Trem2Y38C/Y38C and Trem2−/− mice showed significant reduction in synaptophysin but not in PSD-95 protein levels. c,d PSD95 and synaptophysin protein levels were significantly reduced in hippocampi of Trem2Y38C/Y38C and Trem2−/− mice. e Comparison of PSD95 and synaptophysin protein expression in hippocampal lysates of WT, Trem2Y38C/Y38C and Trem2−/− mice at P20 and 6 months. Comparing P20 to 6 months, PSD95 levels increase significantly (black asterisk) in WT mice (black), while both Trem2Y38C/Y38C (green) and Trem2−/− (red) mice show comparatively less increase resulting in reduced PSD95 levels at 6 months as compared to WT. Synaptophysin levels were maintained in WT mice (black) and Trem2Y38C/Y38C (green), while Trem2−/− (red) mice showed significantly lower levels at 6 months as compared to P20 (red asterisk) indicating loss of presynaptic elements in adult mice. Sample sizes for each group: N = 6–8 (3–4 each, males and females). Data are shown as mean ± SEM. b,d One-way ANOVA with Tukey’s post hoc test; *P < 0.05, **P < 0.01, ***P < 0.001, **** P < 0.0001. e Two-way ANOVA with Bonferroni’s post hoc test; *P < 0.05, WT mice at 6 months versus P20 (black) and Trem2−/− mice at 6 months versus P20 (red)

Back to article page
\