Fig. 4

Tau mutations mimicking post-translational modifications to T231 and K274/281 cause mitochondrial fragmentation. a-d Representative images of mitochondria from PLM neuron cell bodies showing different classes of fragmentation. Each panel is color-coded to the data in panel (e), as indicated. Asterisks denote overt swollen mitochondria resulting from excessive fragmentation. Labeling was via mito-mKeima, imaged on a single channel specific for mitochondria. e Data from day 10 adults presented in a more granular fashion, with individual cells assigned a category as depicted in panels (a-d). N = 30 ± 5 cells from separate animals, performed in two independent biological replicates. Statistical analysis was by Wilcoxon signed-rank test, with *P < 0.05 compared to Dendra2