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Fig. 2 | Molecular Neurodegeneration

Fig. 2

From: Aging-relevant human basal forebrain cholinergic neurons as a cell model for Alzheimer’s disease

Fig. 2

Electrophysiological properties of hiBFCNs. a Representative AP waveforms recorded under the current-clamp mode for a hiBFCN at 49 dpi. The black traces represent the precondition sweep and the sweep at the highest frequency. The red trace represents the sweep immediately above the threshold. A total of 44 out of 54 recorded cells fired APs. b-d Intrinsic properties of the recorded neurons from the indicated samples at between 49 and 55 dpi (mean ± SEM; n = 5 neurons for NL1, and n = 13 neurons for NL2). e-l Spiking characteristics of the APs for the indicated samples at between 49 and 55 dpi (mean ± SEM; n = 5 neurons for NL1, and n = 13 neurons for NL2). m Representative sodium and potassium currents under voltage-clamp mode for a recorded hiBFCNs at 55 dpi. An enlarged view of the boxed region is shown on the right. n-p Characteristics of ions currents for the recorded samples (mean ± SEM; n = 5 neurons for NL1, and n = 13 neurons for NL2). INa, sodium current; IA, A-type potassium current; Id, delayed-rectifier potassium currents. q A representative voltage sag evoked by hyperpolarizing currents for a hiBFCN at 55 dpi. r Quantification of sag voltages for the recorded samples (mean ± SEM; n = 4 neurons for NL1, and n = 13 neurons for NL2). s Representative Ih evoked by hyperpolarizing voltage steps for a hiBFCN at 55 dpi. t Quantification of the Ih currents for the recorded samples (mean ± SEM; n = 4 neurons for NL1, and n = 12 neurons for NL2)

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