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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Induction of autophagy mitigates TDP-43 pathology and translational repression of neurofilament mRNAs in mouse models of ALS/FTD

Fig. 1

IMS-088 treatment Inhibits NF-κB activation in vitro. a Stably transfected BV2 cells with pGL4.32[luc2P/NF-κB–RE/Hygro] plasmid carrying 5 copies of an NF-κB response element that drives transcription of the luciferase reporter gene luc2P were used for the experiment. LPS treatment was given to these cells with bacterial LPS (500 ng/ml) for 2 h. Post-treatment of LPS stimulated cells were treated with varying concentrations of IMS-088 or WFA in LPS containing media for 2 h (n = 3; one way ANOVA; Tukey multiple comparison test). b Cell death assay seen in BV2 cell by addition of lipopolysaccharide (LPS), Withaferin A (WFA) or IMS-088 in dimethyl sulfoxide (DMSO) at varying concentrations (one way ANOVA; Bonferroni’s multiple comparison test; n = 6). c Stably transfected NSC-34 cells with pGL4.32[luc2P/NF-κB–RE/Hygro] plasmid carrying 5 copies of an NF-κB response element were used for the experiment. TNF-α (40 ng/ml) treatment was given to the cells to induced NF-κB luciferase activity. TNF-α stimulated cells were post-treated with 1 μM of IMS-088 or WFA in TNF-α containing media (n = 4, one way ANOVA; Tukey’s multiple comparison test). d Cell death difference observed in NSC-34 cell by addition of TNF-α, Withaferin A (WFA) or IMS-088 in dimethyl sulfoxide (DMSO) (n = 4; one way ANOVA; Bonferroni’s multiple comparison test). (#P < 0.0001 *** p < 0.0001, ** p < 0.001, * p < 0.05)

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