Fig. 3

C3aR1 knock-out impairs phagocytosis in primary microglia. a, Representative images of latex bead phagocytosis assay on WT primary microglia (top) or C3aR1 knockout (KO) primary microglia (bottom, immunolabelled with IBA1 antibody in red and DAPI in blue). Scale bar = 50 μm. b, Left panel: Quantification of C3aR1 KO microglial cells showing at least one latex bead in their cell body (percentage of WT) in the non-treated condition; Right panel: quantification of C3aR1 KO microglial cells showing at least one latex bead in their cell body (% control) after treatment with the different peptides (1 μM TLQP-21 or C3aSA, for 1 h). n = 18 photomicrographs/group from n = 4–5 wells with N = 2 independent experiments (data presented are the average of both experiments). Error bars represent means ± SEM. Statistical analyses were performed using a Student t-test (left histogram) or a One-Way ANOVA followed by a Tukey’s post-hoc test (right histogram), ***p < 0.001