Fig. 4

TLQP-21 induced differentially expressed genes (DEGs) are related to cell migration and proliferation. a, Volcano plot representations of the DEGs in WT primary microglia treated with TLQP-21 or C3aSA. Red dots represent DEGs at an FDR < 0.05. b, Correlation of the fold changes (FC) of DEGs between WT + TLQP-21 vs WT and WT + C3aSA vs WT comparisons. c, Additional volcano plot representations of the DEGs in primary microglia treated with TLQP-21, C3aSA or TLQP-R21A. d, Tables listing the DEGs (FDR < 0.05) for the WT + C3aSA vs WT comparison (n = 21 DEGs) compared to the first most significant 21 genes in the WT + TLQP-21 vs WT comparison (genes shaded green are similarly regulated in the top 21 by TLQP and by C3aSA (~ 70%). Indicated in bold and red are the significant DEGs with FDR < 0.05. Indicated in green are the genes found in both comparisons (≈70% similarity). e, Heatmap of significantly affected genes in the most significant canonical pathway found in the WT + C3aSA vs WT comparison using Ingenuity Pathway Analysis (IPA) software (“Cellular movement and proliferation” z-score = 1.091, p-value = 8.05E-05). f, Network modules identified by weighted gene co-expression network analysis (WGCNA). The co-expression network (13 samples in total) was constructed using all WT-related comparisons. Each row and column correspond to a gene. The modules are indicated by the colored bars next to the heat map. Light color in the heat map indicates low topological overlap and progressively darker red represents higher topological overlap. The right histogram represents GO terms after Gene Ontology (GO) analysis. Each significant GO term was grouped according to its parental ontology to underline highly represented functions using REVIGO