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Fig. 7 | Molecular Neurodegeneration

Fig. 7

From: VGF-derived peptide TLQP-21 modulates microglial function through C3aR1 signaling pathways and reduces neuropathology in 5xFAD mice

Fig. 7

Human TLQP-21 and C3aSA similarly activate human microglia. a, Left: The sequence of the human TLQP-21 (hTLQP-21) peptide and a photomicrograph of the human microglial clone 3 cell line (HMC3) immunostained with the microglial marker anti-Iba1, are shown. Right: RT-qPCR quantification of human c-Fos expression after treatment (0 to 180 min) of HMC3 cells with 1 μM of hTLQP-21 or C3aSA, n = 3 per group. b, Representative images (Left) and quantification (Right) of latex bead phagocytosis assay on HMC3 microglia treated with or without 1 μM of hTLQP-21 for 1 h. Scale bar = 50 μm. c, RT-qPCR analysis for the human forms of the top 6 targets identified by the murine primary microglia RNA sequencing after treatment with the murine form of TLQP-21 or C3aSA (see Fig. 4) in HMC3 treated with or without hTLQP-21 or C3aSA for 24 h, n = 4 treated wells per group from 2 independent experiments A Kruskal-Wallis test was used for A, a Student-t-test for B and a One-Way ANOVA followed by a Tukey’s post-hoc for C, *p < 0.05; **p < 0.01; ***p < 0.001

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