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Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer’s disease

Fig. 1

Experimental setup and characterization of disease-specific PrP conformers. A) Scheme of the fractionation of different conformers. Density gradient centrifugation with a 10–45% sucrose step gradient was used to separate the density variants. Centrifugation was carried out at 50,000 rpm and 5 °C. Twenty density fractions were taken from top to bottom (lighter to dense) and used for downstream biochemical assays. B) Profile of high-density PrP (HDP) oligomer occurrence in cortical isolates of rpAD, spAD, sCJD patients (+ve con) and non-demented controls (ND. Con). In contrast to spAD, HDP oligomers were detected in density fractions 12 to 17 in rpAD, thus overlapping with HDPs isolated from sCJD samples

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