Fig. 1From: Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer’s diseaseExperimental setup and characterization of disease-specific PrP conformers. A) Scheme of the fractionation of different conformers. Density gradient centrifugation with a 10–45% sucrose step gradient was used to separate the density variants. Centrifugation was carried out at 50,000 rpm and 5 °C. Twenty density fractions were taken from top to bottom (lighter to dense) and used for downstream biochemical assays. B) Profile of high-density PrP (HDP) oligomer occurrence in cortical isolates of rpAD, spAD, sCJD patients (+ve con) and non-demented controls (ND. Con). In contrast to spAD, HDP oligomers were detected in density fractions 12 to 17 in rpAD, thus overlapping with HDPs isolated from sCJD samplesBack to article page