Prion protein oligomers cause neuronal cytoskeletal damage in rapidly progressive Alzheimer’s disease

Table 1 HDP-binding interactors in rpAD high-density fractions identified by mass spectrometry assisted co-immunoprecipitation using anti-PrP antibody

No.	IDs	UniProt Acc. No.	Protein name	Occurrence	Sub-cellular location	Disease Relevance
1	KCC2B	Q13554	Calcium/calmodulin-dependent protein kinase type II subunit beta	rpAD-F12, sCJD-MM1-F13, 17, sCJD-MM2-F16, 17, sCJD-VV2-F16, 17	C, Ck, Ce, Sr, Sy	Alzheimer’s disease [59]
2	KCC2D	Q13557	Calcium/calmodulin-dependent protein kinase type II subunit delta	rpAD-F12, sCJD-MM1-F13, 17, sCJD-MM2-F16, 17, sCJD-VV2-F16, 17	Cm, Sl	Alzheimer’s disease [59]
3	EPDR1	Q9UM22	Mammalian ependymin-related protein 1	rpAD-F12, sCJD-MM1-F13–16, sCJD-MM2-F13–16, sCJD-VV2-F12, 14, 15	S	Unknown
4	DIRA2	Q96HU8	GTP-binding protein Di-Ras2	rpAD-F13, 15, sCJD-VV2-F17	Cm	Unknown
5	G2L2	Q8NHY3	GAS2-like protein 2	rpAD-F16	C, Ck	Unknown
6	1433S	P31947	14–3-3 protein sigma	rpAD-F17, sCJD-VV2-F17	C, Nu, S	Parkinson’s disease [60], Alzheimer’s disease, Creutzfeldt Jakob disease [61], Epilepsy [62]

F12 to F17: HDF-pool 12 to 17. Ce: centrosome, Sy: synapse, Sr: sarcoplasmic reticulum, C: cytoplasm, Ck: cytoskeleton, Nu: nucleus, S: secreted, Cm: cell membrane, Sl: sarcolemma, The localization of proteins and accession number are assigned as in the ExPASy protein database and Uniprot data base, respectively. Disease relevance of the HDP-interacting proteins was identified using Uniprot database search, as well

ISSN: 1750-1326