Skip to main content
Fig. 1 | Molecular Neurodegeneration

Fig. 1

From: Clusterin secreted from astrocyte promotes excitatory synaptic transmission and ameliorates Alzheimer’s disease neuropathology

Fig. 1

Clu is expressed in astrocyte and targeted to the excitatory synaptic sites. a Immunofluorescence staining of hippocampal area CA1 of a Aldh1L1-EGFP mouse showing co-localization of endogenous Clu with EGFP-marked and GFAP-positive astrocytes. b Higher magnification confocal images showing abundant co-localization of Clu with excitatory synaptic structures marked by vGlut1 but not with inhibitory synapses marked by GAD67. c Immunofluorescence staining showing astroglial expression of Clu or GFP in Clu knockout mice 3 weeks after AAV-GFAP-Clu or AAV-GFAP-GFP infection. d Co-immunofluorescence staining of Clu and vGlut1 and high resolution confocal imaging of Clu KO mice infected with AAV-GFAP-Clu, showing co-localization of Clu puncta with vGlut1. Non-infected Clu KO (Clu) mice were used as controls. Scale bars, 20 μm (a), 5 μm (c) and 1 μm (b) & (d)

Back to article page