Fig. 2

Impaired excitatory neurotransmission in the hippocampus of Clu null mice. a mEPSC sample traces from WT and Clu KO neurons voltage clamped at − 65 mV (scale bar, 20pA/2 s). b Cumulative distribution of inter-event interval from all mEPSCs events of 16 WT and 17 KO neurons (left) and average mEPSC frequency (right). c Cumulative distribution of amplitude of all neurons (left) and average mEPSC amplitude (n = 16 for WT and n = 17 for KO). d mIPSC sample traces from WT and KO neurons voltage clamped at 10 mV (scale bar, 20pA/2 s). e Cumulative distributions of inter-event interval from all mIPSCs events of 11 WT and 10 KO neurons (left) and average mIPSC frequency (right). f Cumulative distribution of amplitude of all neurons (left) and average mEPSC amplitude (n = 11 for WT and n = 10 for KO). g Relationship between fEPSP slope and stimulating current intensity in WT and KO hippocampi showing a significant decrease in CA3-CA1 excitatory transmission (n = 14 for both WT and KO). h Average paired pulse ratio (PPR) of fEPSP slope in CA3-CA1 synapse showing increased PPR in KO compared to WT (n = 17 for WT, n = 13 for KO). All data are presented as mean ± SEM. Average number of animals tested: N = 3 per group for whole cell recording and N = 4 per group for field recording.*p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. Student’s t-test (b), (c), (e), (f); Two-way ANOVA (g) & (h)