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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: Clusterin secreted from astrocyte promotes excitatory synaptic transmission and ameliorates Alzheimer’s disease neuropathology

Fig. 6

Astrocytic expression of Clu rescues synaptic deficit in Clu KO mice. a Western blotting of Clu expression in hippocampal tissues of WT and Clu KO mice injected with AAV-GFAP-GFP or AAV-GFAP-Clu. Tub: Tubulin loading control. b mEPSC sample traces from hippocampal CA1 pyramidal neurons of KO mice injected with AAV-GFAP-GFP (KO;GFP) or AAV-GFAP-Clu (KO;Clu). Scale bar, 20 pA/1 s. c Cumulative distribution of inter-event interval (left) from all mEPSCs events of 19 neurons in KO mice each injected with AAV-GFAP-GFP or AAV-GFAP-Clu. d Cumulative distribution of amplitude (left) and average mEPSC amplitude of KO neurons infected with GFP or Clu viruses (n = 19 each). e Representative dendritic spines of Thy1-GFP-labeled dendrites of area CA1 of hippocampal pyramidal neurons of four-month-old Clu KO injected with AAV-GFAP-GFP (KO;GFP) or AAV-GFAP-Clu (KO;Clu). IMARIS softward was used to reconstruct the 3D rendering of the spines on dendrites based on confocal images of GFP-positive neurons and to classify the four spine types (mushroom, stubby, long-thin, and filopodia). Scale bar, 2 μm. f Total spine density in KO;GFP and KO;Clu mouse brains. g Density of each of the four spine types in secondary apical dendrites. Dendritic segments were derived from 5 random neurons per mouse/ 4 animals for each group. Number of animals tested for mEPSCs: N = 3 per group. All data are presented as mean ± SEM. *p < 0.05; p < 0.01; p < 0.001 (Student’s t test)

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