Fig. 4

Retinal neurons in different strains of young and aged mice. Retinal sections from young (3-5 m) and aged (10-12 m) Socs3^fl/fl, LysMCre-Socs3^fl/fl, Cx3cr1^gfp/gfp and DKO mice were stained with secretagogin (a), PKCα (c) and GABA (e) and imaged by Dmi8 fluorescence microscopy. Sections in (a) and (c) were count-stained for DAPI. Quantitative analysis of secretagogin⁺ (b) and PKCα⁺ cells (d). (f) Quantitative analysis of GABAergic cells in both GCL and INL (white arrows in e). Scale bar: 25 μm. Mean ± SD, N = 3 ~ 5 mice, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; Two-way ANOVA followed by Tukey’s test. GCL – ganglion cell layer; IPL – inner plexiform layer; INL – inner nuclear layer; OPL – outer plexiform layer; ONL – outer nuclear layer