Fig. 9

The effect of microglia from Socs3^fl/fl and DKO mice on photoreceptor survival in retinal explant cultures. Brain microglia were cultured from Socs3^fl/fl and DKO mice. Retinal explants were from Socs3^fl/fl mice. Retinal explants were co-cultured with different types of microglia for 24 h. The flatmounts of retinal explants were then stained for cone arrestin and imaged by Dmi8 fluorescence microscopy. a Schematic diagram showing study design. b Representative images showing cone arrestin⁺ cells in retinal explants in different treatment groups. c Quantitative data of cone arrestin⁺ cells in different groups. The number of cone arrestin⁺ cells in different treatment groups was compared to retinal explants without microglia co-culture. Mean ± SD. N = 3 ~ 6 explants. * P < 0.05, ** P < 0.01, *** P < 0.001. Two-way ANOVA followed by Tukey’s multiple comparisons. Scale bar: 50 μm