Fig. 3

Synaptic plasticity. a and b Example recordings of mEPSCs prior to and after DPP (10 depolarising pulses of 3 s in duration, every 9 s, from − 84 to + 16 mV) for C9ORF72^RE (1. grey and 2. black, respectively; scale bar; 20 pA, 5 s) and C9ORF72^RE-Δ (1. light red and 2. red, respectively; scale bar; 10 pA, 5 s) neurons. c, d Individual mEPSC amplitude plots for DPP experiments performed upon C9ORF72^RE and C9ORF72^RE-Δ neurons, respectively. For C9ORF72^RE neurons, mEPSCs before (1) and after DPP (2) are represented in grey and black, respectively. For C9ORF72^RE-Δ neurons, mEPSCs before (1) and after DPP (2) are represented in light red and red, respectively. Note the lack of potentiation in C9ORF72^RE, but not C9ORF72^RE-Δ, neurons. e Cumulative probability plots of mEPSC amplitudes for the initial control period (1) and after DPP (2) of the data shown in C and D. Significance of shift of mEPSC data (Kolmogorov–Smirnov test); C9ORF72^RE, p = 0.325; C9ORF72^RE-Δ, p < 0.001. f Left, mean mEPSCs for data shown in B (C9ORF72^RE) and C (C9ORF72^RE-Δ) for initial control period (1) and post DPP (2). Scale bars; 5 pA, 5 ms. Right, mean scaled mEPSCs. g Mean ± s.e.m. fold increase of mEPSC amplitude 10 minutes post-DPP from initial control period for control (n = 17), C9–1 (n = 14), C9–1Δ (n = 13), C9–2 (n = 4) and C9–2Δ (n = 10). Example traces and data for the control line are presented in Figure S10. Significance determined by unpaired t-tests (C9ORF72^RE versus C9ORF72^RE-Δ) and one-way ANOVA with Bonferroni’s post hoc test (control versus C9ORF72^RE )