Fig. 3
Altered miniature synaptic responses and levels of GABAAR subunits in the OB of APP/PS1 mice. a Representative mEPSC and mIPSC traces. b Significantly increased mIPSC amplitude in the OB of APP/PS1 compared to WT mice (n = 19 cells from 3 WT mice and 16 cells from 3 APP/PS1 mice, p = 0.0003). c Significantly decreased mEPSC frequency in the OB of APP/PS1 compared to WT mice (n = 13 cells from 3 WT mice and n = 10 cells from 3 APP/PS1 mice, p = 0.03). d-e Representative immunoblots and quantification of excitation- and inhibition-related protein levels in the OB (n = 3–9 per genotype, GABAAR β3: p = 0.03; GABAAR α1: p = 0.03). f-i Representative images and statistical analysis of immunofluorescent staining of GABAAR α1 and β3 subunits in the OB of WT and APP/PS1 mice at 3–5 months (n = 3 slices/mouse, 3 mice for WT and 4 mice for APP/PS1; GABAARα1: p = 0.036 for MCL, p = 0.032 for EPL, p = 0.021 for GL; GABAARβ3: p = 0.021 for MCL, p = 0.019 for EPL, p = 0.033 for GL). j-k Representative images and statistical analysis of immunofluorescent staining of GABAAR α5 subunit (n = 3 sections/mouse, 3 mice for WT and 4 mice for APP/PS1; p = 0.793 for MCL, p = 0.529 for EPL, p = 0.597 for GL). Scale bar: 100 μm. Values represent mean ± SEM. Two-sample t-test. *p < 0.05; ***p < 0.001