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Table 2 Details of the primary antibodies used in the study

From: Cholesterol alters mitophagy by impairing optineurin recruitment and lysosomal clearance in Alzheimer’s disease

Antibody Company Cat. Number; RRID WB dilution IF/IHC dilution
ACTB Sigma-Aldrich A3854; RRID:AB_262011 1:40,000  
CTSD Santa Cruz Biotech. sc-6486; RRID:AB_637896 1:500  
CYC BD Biosciences 556,433; RRID:AB_396417 1:1000  
CYC BD Biosciences 556,432; RRID:AB_396416   1:300
GAPDH Abcam ab8245; RRID:AB_2107448 1:5000  
GST Santa Cruz Biotech. sc-374,171; RRID:AB_11008206 1:1000 1:300
HDAC6 Santa Cruz Biotech. sc-5258; RRID:AB_2116625   1:300
LAMP2 Abcam ab13524; RRID:AB_2134736   1:300
LC3B Cell Signaling #2775S; RRID:AB_915950 1:1000 1:300
MFN2 Santa Cruz Biotech. sc-50,331; RRID:AB_2142754 1:500  
OPTN Santa Cruz Biotech. sc-166,576; RRID:AB_2156554 1:1000 1:300
SQSTM1 Abcam ab91526; RRID:AB_2050336 1:1000 1:500
PARL Aviva Systems Biology ARP44851_P050; RRID:AB_1294596 1:1000  
Parkin Abcam Ab77924; RRID:AB_1566559 1:1000 1:1000
PDH Abcam ab110330; RRID:AB_10858459 1:1000  
PGC-1α Millipore ST1202; RRID:AB_2237237 1:1000  
PINK1 Abcam ab23707; RRID:AB_447627 1:1000 1:500
PINK1 Novus Biotech. BC100–494; RRID:AB_10127658 1:1000  
RHOT1 Biorbyt orb165560 1:1000  
p-TBK1 (Ser172) Cell Signaling #5483; RRID:AB_10693472 1:1000  
TBK1 GeneTex GTX113057; RRID:AB_11174793 1:1000  
TFAM GeneTex GTX103231; RRID:AB_11176720 1:1000  
TOMM20 Santa Cruz Biotech. sc-11,415; RRID:AB_2207533 1:500 1:300
Ub Santa Cruz Biotech. sc-9133; RRID:AB_2180553 1:500  
K63-Ub Affymetrix 14–6077 1:500 1:300
ULK1 Santa Cruz Biotech. sc-33,182; RRID:AB_2214706 1:250  
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