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Fig. 6 | Molecular Neurodegeneration

Fig. 6

From: High dose expression of heme oxigenase-1 induces retinal degeneration through ER stress-related DDIT3

Fig. 6

HMOX1 positively regulates Ddit3 expression. a Heat-map of differentially expressed genes from the cluster of positive neuron cell death regulator genes based on the GO analysis of the RNA seq data. Results from albino neural retinas after LD for 1 day (n = 5, p < 0.05, one-way ANOVA) (NL: normal light control). b q-RT-PCR data showing expression of Hmox1 and ER stress-related genes in albino neural retinas after 1 day of LD (Error bars: SD; n = 3, Student T-test). c, d Western blots and corresponding quantification using anti-HMOX1 and anti-DDIT3 in neural retinas after LD for 1 day (Error bars: SD; n = 3, one-way ANOVA). e-h Western blots of anti-GFP and anti-DDIT3 in neural retinas infected by a low (n = 3) or high dose (n = 3) of AAV8-GFP (e) and quantitative bar graphs according to the immunoblots (f) (Error bars: SD; one-way ANOVA). g, h Western blots of anti-HMOX1 and anti-DDIT3 in retinas infected by a low (n = 3) or high (n = 3) dose of AAV-HMOX1 (g) and quantitative bar graphs of expression of HMOX1 and DDIT3 (h) (Error bars: SD; one-way ANOVA). * or ** indicates p < 0.05 or p < 0.01

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