Fig. 1

ApoE4 increases cPLA2 but not iPLA2 expression in mouse primary astrocytes. a, cPLA2 mRNA levels in primary astrocytes from APOE3-TR and APOE4-TR mice. b, cPLA2, and phosphorylated cPLA2 (p-cPLA2) protein levels in primary astrocytes from APOE3-TR and ApoE4-TR mice (left) were detected by WB. c, iPLA2 mRNA levels in primary astrocytes from ApoE3-TR and ApoE4-TR mice. d, iPLA2 protein levels in primary astrocyte cultures from APOE3-TR and ApoE4-TR mice were detected by WB. e, f, Primary astrocytes from APOE3-TR and ApoE4-TR mice were incubated with ³H-labelled AA (e) or ¹⁴C-labeled DHA (f) for 24 h, followed by induction by 100 nM ATP for 15 min. The efflux of ³H-AA (e) and ¹⁴C-DHA (f) from cells to media was measured by scintillation counting. g, h, Primary astrocytes from C57BL/6 wild type mice were labeled with ³H-AA (g) or ¹⁴C-DHA (h) for 24 h and then treated with recombinant ApoE3 or ApoE4 for 24 h, followed by induction with 100 nM ATP for 15 min. ³H-AA (g) and ¹⁴C-DHA (h) efflux were measured by scintillation counting. WB: western blot