Fig. 8

Inhibition of cPLA2 reduces ApoE4 mediated up-regulation of LTB4, ROS, and iNOS levels. a-c ApoE3 and ApoE4 primary astrocytes from mice were pre-treated with cPLA2 inhibitor- pyrrophenone (500 nM) for 30 min and then treated with medium or TNFα plus IFNγ together for 18 h. Total and p-cPLA2 in the cell lysate were detected by WB (a). LTB4 levels in the culture medium were measured by the assay kit (b). iNOS expression in cell lysate was detected by WB (c). d, ApoE3, and ApoE4 primary astrocyte were pre-treated with cPLA2 inhibitor-pyrrophenone (1 μM) for 30 min and then treated with medium or TNFα plus IFNγ together for 24 h. The ROS level were detected by the DCFDA probe. e, f, ApoE4 primary astrocytes were transfected with cPLA2 siRNA (10 nM) or Non-target (NT) siRNA (10 nM) for 48 h and then treated with medium or TNFα plus IFNγ together for 24 h. cPLA2 protein levels in cell lysate were detected by WB (e). LTB4 levels in the culture medium were measured by the assay kit (f). Two-way ANOVA was used in a, c, and d for group comparisons. WB: Western blot