Fig. 9From: RETRACTED ARTICLE: Calcium-dependent cytosolic phospholipase A2 activation is implicated in neuroinflammation and oxidative stress associated with ApoE4ApoE4 and Aβ42 induced cPLA2 activation in human postmortem frontal cortical synaptosomes. a, Synaptosomes were incubated in oxygenated Kreb’s Ringer with different reagents: rApoE3, rApE4, Aβ42, Aβ42/rApoE4 for 30 min, followed by 15 min incubation with Kreb’s-Ringer (control), TNFα/IFNγ or ceramide-1-phosphate, respectively. The synaptosomes were harvested and homogenized, and the membrane and cytosolic fractions of synaptosomes were isolated by centrifugation. cPLA2 was enriched in both fractions by immunoprecipitation with anti-cPLA2 antibodies, and the p-cPLA2 and total cPLA2 were detected by western blot. b-d, Densitometric quantification of blotting shown in ABack to article page