Fig. 9
From: Parkinson mice show functional and molecular changes in the gut long before motoric disease onset
Ingenuity pathway analysis (IPA). IPA shows the relationships between altered miRNAs in the myenteric plexus (MP) of the large intestine (LI) from pre-symptomatic (ps)A30P mice compared with wild type (WT) controls and their target proteins. IPA functional pathway analysis was used to predict the top transcriptional regulators from differentially expressed proteins in murine PD models. miRNA target (protein) filter pairing analysis was done in order to identify co-regulated miRNAs/target pairs. We identified 77 protein-miRNA interactions which involved 25 dysregulated proteins and 31 miRNAs and eight protein-protein interactions. These predictions are based on Ingenuity Pathway Knowledge Base (IPKB). Nefl is targeted by let 7i-5p, miR-10a-5p, miR-22-3p, miR-23a-3p, miR-30c-5p, miR-99a-5p, miR-125a-5p, miR-126-3p, miR-126-5p, and miR-181a-5p; Vamp2 is targeted by let-7i-5p, miR-10a-5p, miR-22-3p, miR-30c-5p, miR-99a-5p, miR-135a-5p, miR-338-3p, miR-350-3p, miR-377-3p, and miR-495-3p; Calb2 is targeted by miR-17-5p, miR-22-3p, miR-30c-5p, and miR-495-3p