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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Plaque associated microglia hyper-secrete extracellular vesicles and accelerate tau propagation in a humanized APP mouse model

Fig. 4

In vivo imaging of EV secretion from microglia after lentiviral microglia-specific expression of mE-CD9. a. Schematic of mE-CD9 lentiviral construct. b. Representative images of mE-CD9 lentivirus transduction in WT and AppNL-G-F microglia following injection and 10-day incubation. c. Representative images of individual microglia and released mE-CD9+ particles showing FSB, Mac2, and mE-CD9. d. Unbiased quantification of mE-CD9+ voxels surrounding individual microglia (n = 12 microglia per group from 3 mice per group). e. Regression plot of Mac2 staining intensity versus the number of mE-CD9 particles released by individual microglia. Representative images displayed in a-e are a mix of male and female mice. All values displayed in a-e represent the mean ± SEM for a minimum of 3 animals per group. Graphs comparing values across all 4 groups were analyzed via 2-way ANOVA with Tukey post-hoc analysis for individual comparisons. Graphs comparing two groups were analyzed via unpaired t-test. ** p < 0.01, *** p < 0.001, between indicated groups

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