Fig. 4

Experimental design and dosing paradigm of the chronic in vivo MLi-2 proteomics experiment. a Schematic of experimental design for the chronic MLi-2 proteomics experiment. b 10-Day cohort animals observed no weight changes (male and female body weight trends are shown separated within genotypes as square and circle points, respectively) and consumed ~ 4 g of chow per day c. Daily dose of MLi-2 was calculated by body weight and food intake and showed that animals on average received ~ 65 mg/kg/day of drug d. e 10-Week cohort animals grew 3-8 g in body weight over the course of 10 weeks (shown as % weight change), with an average food intake of 4 g f and hit the 60 mg/kg/day mark each week g