Fig. 5

LRRK2 and S106 Rab12 phosphorylation following chronic MLi-2 treatment. WT, G2019S LRRK2 KI, and LRRK2 KO mice were fed vehicle or MLi-2-containing chow over the course of 10 days or 10 weeks a. LRRK2 inhibition is maintained between the short-term (10-day) and long-term (10-week) chronic treatment as shown by decrease in pS935 levels d, e and S1292 LRRK2 dephosphorylation b, c to levels comparable to WT controls. A decrease in total LRRK2 levels is observed in peripheral tissues with chronic treatment in by 10 days f and is exacerbated in the 10-week treatment g. S106 Rab12 phosphorylation levels show a significant decrease with treatment to levels comparable to WT tissue h, i. One-way ANOVA with Tukey’s post hoc; n = 6; ****P < 0.0001, ***P < 0.0002, **P < 0.005, *P < 0.05; b F (11, 60) = 146.8, c F (11, 60) = 178.7, d F (11, 60) = 158.0, e F (11, 60) = 265.9, f F (11, 60) = 75.97, g F (11, 60) = 271.5, h F = (11, 60) = 65.78, i F (11, 60) = 59.67