Fig. 7

Proteomic analysis of changes in brain and lung between G2019S LRRK2 MLi-2 and G2019S vehicle 10-week treated mouse cohorts. A volcano plot from total proteomics of whole brain tissue comparing the G2019S chronic MLi-2 cohort versus G2019S KI vehicle controls a. Modest upregulation was observed in mitochondrial proteins with treatment, specifically proteins of the electron transport chain a. Heatmap visualizes changes in phosphopeptides with treatment highlighted proteins involved in vesicular trafficking and postsynaptic regulation on a z score scale b. GO:CC term enrichment bubble plots were generated from the significant hits of the total and phospho-proteomics showing high enrichment for mitochondrial compartments, as well as proteins associated with postsynaptic function, respectively c-d. Volcano plots from total and phospho-proteomics of whole lung homogenates comparing treated versus untreated G2019S KI mice e-f. Volcano plots key: grey = non-significant, blue = < 0.05 p-value, green = > 1.4x log fold change, red = < 0.05 p-value and > 1.4x log fold change