Fig. 1
From: The CD33 short isoform is a gain-of-function variant that enhances Aβ1–42 phagocytosis in microglia
A gain-of-function revealed for mouse primary microglia expressing hCD33m. a-c A competitive flow cytometry-based phagocytosis assay between primary hCD33M+ (red) and WT (black) microglia, showing the (a) gating strategy, (b) representative plots for the uptake of aggregated fluorescent Aβ1–42 in the absence (solid line) or presence (dashed line) of cytochalasin-D by WT (black) and hCD33M+ (red) microglia, and (c) quantification of the MFI . % Phagocytosis represents the cytochalasin-D subtracted MFI values referenced to the average of the WT microglia set to 100%. (N = 5) (d-f) A competitive flow cytometry-based phagocytosis assay between primary hCD33m+ (blue) and WT (black) microglia, showing the (d) gating strategy, (e) representative plots for uptake of aggregated fluorescent Aβ1–42 in the absence (solid line) or presence (dashed line) of cytochalasin-D by WT (black) and hCD33m+ (blue) microglia, and (f) quantification of the MFI. % Phagocytosis represents the cytochalasin-D subtracted MFI values referenced to the average of the WT microglia set to 100%. (N = 6) (g) Results from a competitive flow cytometry-based phagocytosis assay between primary hCD33m+ (blue) and WT (black) microglia on a mCD33−/+ and mCD33−/− background. % Phagocytosis represents the cytochalasin-D subtracted MFI values referenced to the average of the hCD33m− microglia set to 100%. (N = 3)