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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: The CD33 short isoform is a gain-of-function variant that enhances Aβ1–42 phagocytosis in microglia

Fig. 4

Pathway analysis reveals early-activating response elements upregulated in hCD33m microglia. a Cluster 0 from Experiment, a cluster enriched with immediate early genes such as Fos. b Comparison of the Fos gene single cell expression (left) and the entire Fos-regulon expression consisting of the dominating transcription factors, regulators and their downstream target genes. c SCENIC Heatmap of the major regulons in each identified cluster. d Schematic showing the Atf5 regulator (purple) in the Fos-regulon and the target genes that it regulates (yellow), with the highly ranked/motif enriched targets in green. e Representative RNAscope images of cells positive for the microglia marker Fcrls (green), nuclear stain DAPI (cyan) and mRNA signals from either Fos (upper) or Klf2 (lower) microglia subpopulations (red). Quantification of Fos and Klf2 microglia cell density in the cortex and hippocampus of control and hCD33m+ isoform tissue. (N = 5 for each group) f Quantification of the number of microglia (Fcrls+) in brain WT and hCD33m+ mice. (N = 5 for each group) g Alluvial plot of the top pathways per cluster, band thickness corresponds to the number of genes associated with each pathway

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