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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Adiponectin-mimetic novel nonapeptide rescues aberrant neuronal metabolic-associated memory deficits in Alzheimer’s disease

Fig. 3

Os-pep rescued neuronal insulin resistance via AdipoR1/AMPK signaling in vitro and in vivo AD models and Adipo−/− mice. (a-c) Immunoblotting and quantification of p-IRS-1 (Ser 636)/ total-IRS-1 and p-IRS-1 (Ser 312)/total-RS-1 levels in the AβO, APP/PS1 and Adipo−/− mice. The data are expressed as the means ± SEM for the indicated proteins in vivo (n = 8 mice/group) and the number of independent experiments = 3. Significance = **p < 0.01, ***p < 0.001; ###p < 0.001; One-way ANOVA followed by Turkey’s post hoc test. ns, indicated that there is no significant difference among the control, sham and Os-pep treated groups (d) Double immunofluorescence staining for Aβ (FITC, green) and p-IRS-1 (Ser 636) (TRITC, red) in the cortex and hippocampus of APP/PS1 mice. The data are expressed as the means ± SEM for n = 5 mice/group, and the number of independent experiments = 3. Magnification 40x and magnified 40X. Scale bar = 50 μm and 20 μm respectively. Significance = **p < 0.01, ***p < 0.001; #p < 0.05, ##p < 0.01, ###p < 0.001; One-way ANOVA followed by Turkey’s post hoc test. (e) Immunoblotting and quantification of p-IRS-1 (Ser 636)/total-IRS-1 and p-IRS-1 (Ser 312)/total-IRS-1 levels in the human APPswe/ind-overexpressed SH-SY5Y cells transfected with the AdipoR1 siRNA and treated with Os-pep (10 μM). The data are expressed as the means ± SEM for the indicated proteins in vitro (n = 5/group), and the number of independent experiments = 3. Significance = ***p < 0.001; ###p < 0.001; Two-way ANOVA followed by Bonferroni’s multiple comparisons test

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