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Fig. 4 | Molecular Neurodegeneration

Fig. 4

From: Adiponectin-mimetic novel nonapeptide rescues aberrant neuronal metabolic-associated memory deficits in Alzheimer’s disease

Fig. 4

Os-pep activated downstream insulin signaling via AdipoR1/AMPK signaling in vitro and in vivo AD models and Adipo−/− mice. (a-c) Immunoblotting and quantification of p-PI3K/total-PI3K, p-Akt/total-Akt and p-GSK3β (Ser 9)/total-GSK3β levels in the AβO-treated, APP/PS1 and Adipo−/− mice. The data are expressed as the means ± SEM for the indicated proteins in vivo (n = 8 mice/group) and the number of independent experiments = 3. Significance = ***p < 0.001; ###p < 0.001; One-way ANOVA followed by Turkey’s post hoc test. ns, indicated that there is no significant difference among the control, sham and Os-pep treated groups (d) Immunoblotting and quantification of p-AMPK/total-AMPK, p-IRS-1 (Tyr632)/total-IRS-1, p-PI3K/total-PI3K, p-Akt/total-Akt and p-GSK3β (Ser 9)/total-GSK3β levels in APPswe/ind-overexpressed SH-SY5Y cells transfected with the AMPK siRNA and treated with Os-pep (10 μM). The data are expressed as the means ± SEM for the indicated proteins in vitro (n = 5/group), and the number of independent experiments = 3. Significance = *p < 0.05, **p < 0.01 ***p < 0.001; #p < 0.05, ##p < 0.01, ###p < 0.001; Two-way ANOVA followed by Bonferroni’s multiple comparisons test (e) Immunoblotting and quantification of p-AMPK/total-AMPK, p-IRS-1 (Tyr 632)/total-IRS-1, p-PI3K/total-PI3K, p-Akt/total-Akt and p-GSK3β (Ser 9)/total-GSK3β levels in APPswe/ind-overexpressed SH-SY5Y cells exposed to a high insulin (1 μM) concentration (SH-SY5YIR), low insulin concentration (10 nM), Os-pep (10 μM) and Comp. C (10 μM) and transfected with the AdipoR1 siRNA. The data are expressed as the means ± SEM for the indicated proteins in vitro (n = 5/group), and the number of independent experiments = 3. Significance = *p < 0.05, **p < 0.01 ***p < 0.001; #p < 0.05, ##p < 0.01, ###p < 0.001; Two-way ANOVA followed by Bonferroni’s multiple comparisons test

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