Fig. 8
Neuroprotective and pharmacokinetic profile of Os-pep in the in vivo and in vitro studies. (a, panels (a-c)) Apotox-Glo Triplex assays of the cell viability, cytotoxicity and activated caspase3/7 levels in SH-SY5Y and HT22 cells treated with AβO (1 μM) and different concentrations of Os-pep. (a, panels (d-f)) Apotox-Glo Triplex assays of the cell viability, cytotoxicity and activated caspase3/7 levels in SH-SY5Y and HT22 cells transfected with the AdipoR1 siRNA and treated with AβO (1 μM) and Os-pep (10 μM). Data are expressed as means ± SEM for three independent experiments. Significance = *p < 0.05, **p < 0.01 ***p < 0.001; #p < 0.05, ##p < 0.01, ###p < 0.001; One-way ANOVA followed by Turkey’s post hoc test. (b) Immunoblotting and quantification of activated caspase-3 and cleaved PARP-1 levels in the APP/PS1 mice. Data are expressed as the means ± SEM for the indicated proteins (n = 8 mice/group), and the number of independent experiments = 3. The data presented in this figure are normalized to control values. Significance = **p < 0.01; ##p < 0.01; One-way ANOVA followed by Turkey’s post hoc test. (c-e) Pharmacokinetic profile of Os-pep. (c) Stability of Os-pep in mouse plasma. The Os-pep concentration at 0 min is set to 100%. Bars represent SD (standard deviation) (n = 3) for triplicate samples. (d) Confocal microscopy images of the Veh-injected WT and Os-pep-FITC-injected WT mice. The number of mice = 3/group and number of independent experiments = 3. Magnification: 10X. Scale bar = 100 μm. (e) Confocal microscopy images of the bEnd cells exposed to bidistilled water and Os-pep-FITC. The number of independent experiments = 3. Magnified 10X. Scale bar = 100 μm