Fig. 2
From: Oligomerization of Lrrk controls actin severing and α-synuclein neurotoxicity in vivo
Manipulation of Lrrk enhances actin cytoskeletal pathology induced by α-synuclein. a, F-actin staining with fluorescent phalloidin in whole mount brains from flies expressing human α-synuclein shows increased F-actin compared to controls. F-actin is further increased when Lrrk protein is removed in homozygous Lrrke03680 mutants, or mutant Lrrk-G1914S (Lrrk-GS), homologous to the human G2019S LRRK2 mutant, is expressed, as quantified in (b) when compared to flies expressing α-synuclein alone. Scale bar in (a) represents 75 µm. c, Immunofluorescent staining with an antibody to actin demonstrates rod-like inclusions when human α-synuclein is expressed in the fly brain. Numbers of rods are increased when Lrrk is removed or Lrrk-GS is expressed (arrows), as quantified in (d) when compared to flies expressing α-synuclein alone. Scale bar in (c) represents 15 µm. n=6 per genotype. Data are represented as mean ± SD. *p<0.05, **p<0.01,***p<0.005, ANOVA with Bonferroni post-test analysis. Control is nSyb-GAL4, nSybQF2/+. Flies are 10 days old