Fig. 2
Frequency of spontaneous excitatory synaptic transmission is reduced in an age-dependent manner in AppNL-F and AppNL-G-F mice. A) Examples of continuous voltage-clamp recordings from CA1 pyramidal neurones in slices from 9- and 20-month-old wild type, AppNL-F and AppNL-G-F animals illustrating the reduction in frequency of spontaneous EPSCs observed at these ages. Confirmed EPSCs are indicated by the asterisks. B) Frequencies (i) and median amplitudes (ii) of spontaneous EPSCs in wild type and knock-in mice. C) Frequencies (i), median amplitudes (ii) and decay time constants (iii) of miniature EPSCs in wild type and App knock-in mice. Data in panels B and C plotted as mean ± SEM. Sample sizes (animals) are indicated by the numbers inside bars in subpanels i. Sequential Sidak corrected post-hoc comparisons to wild type are indicated by * p < 0·05; *** p < 0·001 (9-months-old: AppNL-G-F p = 0·025; 20-months-old: AppNL-F p = 0·018, AppNL-G-F p = 0·00028)