Fig. 6
Microglial gene expression is upregulated at an earlier plaque pathology stage in AppNL-F than AppNL-G-F mice. Ai) Aif1 (the gene encoding IBA1) gene expression determined by RT-qPCR expressed relative to the housekeeping gene Actg1 for males (left) and females (right). Sequential Sidak post hoc analyses following GLMM are indicated by * p < 0·05; ** p < 0·01; *** p < 0·001; **** p < 0·0001; NS not significant (Males: 9-month-old p = 0·36, 24-month-old p = 0·026; Females: 18-months-old p = 0·0013, 24-month-old p = 0·033). Aii) Sigmoidal fit to Aif1 expression plotted against corresponding pathology score for an individual animal. Bi) Trem2 gene expression relative to the housekeeping gene Actg1, representing a global Trem2 expression in the hippocampus for males (left) and females (right). Sequential Sidak post hoc analyses following GLMM are indicated by *** p < 0·001; **** p < 0·0001 (Males: 9-months-old p = 0·00015, 18-months-old p = 4·0 × 10−7, 24-month-old p < 1 × 10−20; Females: 9-months-old p = 1·5 × 10−5, 18-months-old p = 8·2 × 10−8, 24-months-old p = 6·7 × 10−14). Bii) Sigmoidal fit to Trem2 expression relative to Actg1 plotted against corresponding pathology score for an individual animal. Ci) Trem2 gene expression relative to Aif1 gene expression, representing a per-microglia Trem2 expression for males (left) and females (right). Sequential Sidak post hoc analyses following GLMM are indicated by *p < 0.05; ** p < 0·01; **** p < 0·0001 (Males: 9-months-old p = 0·0020, 18-months-old p = 0·019, 24-months-old p = 1·1 × 10−5; Females: 9-months-old p = 0·026, 18-months-old p = 0·042, 24-months-old p = 0·0025). Cii) Sigmoidal fit to Trem2 expression relative to Aif1 plotted against corresponding pathology score for an individual animal. Sequential Sidak post hoc analyses following GLMM are indicated by * p < 0·05; ** p < 0·01; *** p < 0·001; **** p < 0·0001; NS not significant (p = 0·36). Data in subpanels i within A-C plotted as mean ± SEM. Sample sizes (animals) are indicated within the bars in panel A