Fig. 1

Anti-CCR2 antibody reduces monocyte populations in the blood without affecting cognitive behavior. WT mice received 4 injections of αCCR2 antibody every 4 days, while control mice were untreated. Mice were euthanized 3 days after the 4th injection, and blood was collected. (A) Flow cytometry gating strategy for monocyte (top) and memory CD4⁺ T cell (bottom) populations in the blood of WT animals. (B) Flow cytometry analyses of Ly6G⁻ CD115⁺ myeloid cells (two-tailed Student’s t-test: t₍₁₄₎ = 2.256, *p = 0.0406), (C) Ly6C⁺ myeloid populations (two-tailed Student’s t-test: Ly6C^hi t₍₁₄₎ = 3.764, **p = 0.0021; Ly6C^med t₍₁₄₎ = 2.442, *p = 0.0285), (D) CD4⁺ T cells, and (E) memory CD4⁺ T cell populations in control and αCCR2-injected groups, n = 8 mice per group. (F) Flow cytometry gating strategy and (G) analysis of Tregs in the blood of WT mice, n = 10 mice per group. Data are presented as mean ± s.e.m. *p < 0.05, **p < 0.01. (H-J) Male WT mice received 4–5 injections of αCCR2 antibody every 4 days, while control mice remained untreated. Behavioral testing was carried out during the 4 days following the last injection. (H) T-maze: Willingness to explore a novel environment is presented as percent of time spent by each mouse in a novel arm divided by the total time spent in all three arms (the novel arm and two familiar arms). (I) Spontaneous alternation in the Y maze: The spontaneous alternation behavior of the mice is presented as percent alternation: number of alternations divided by number of possible triads (see Methods). (J) Novel Object Recognition (NOR): The memory recognition is presented as percent time the mouse interacted with the novel object divided by the total time spent with both objects. n = 6 mice per group. Data are presented as mean ± s.e.m. One-way ANOVA was used for the analyses