Fig. 1

Genotype Confirmation, IOP Elevation, and RGC Density Measurements. A Quantitative RT-PCR measurements of Wld^S transgene in genomic DNA from Wld^S null (Wld^S−/−), Wld^S heterozygous (Wld^S+/−), and Wld^S homozygous (Wld^S+/+) mice. Values are normalized against WT animals. B Agarose gel (2%) showing RT-PCR detection in the retina of Wld^S chimeric (a,b) and housekeeping GAPDH (c) transcripts from Wld^S+/+, Wld^S+/−, and WT mice. 1,2 indicates samples extracted from different mice. C Mean intraocular pressure (IOP) in WT (circles) and Wld^S+/+ (squares) mice before (day 0) and following (days ≥1) a single unilateral injection of polystyrene microbeads (gray symbols) or equivalent volume of saline (white symbols). D Microbead injection significantly elevated IOP in all genotypes (WT: 19.64 ± 1.82 vs. 14.81 ± 1.54 mmHg, p < 0.001; Wld^S+/+: 19.48 ± 1.98 vs. 14.23 ± 1.61 mmHg, p < 0.001). E Representative confocal images of whole-mount retinas from WT and Wld^S+/+ saline and microbead injected eyes immunolabeled against RBPMS (grayscale). Scale bar = 25 μm. F When quantified, we did not detect a significant difference in RBPMS+ RGCs regardless of genotype or IOP condition (p = 0.389). Statistics: Student’s t-test (D). One-Way ANOVA Tukey’s Post hoc (F). Data = mean ± SEM