Fig. 5
From: Neuroprotection by WldS depends on retinal ganglion cell type and age in glaucoma
Morphological and Physiological Comparison of αOFF-Sustained RGCs from WT and WldS+/+ Control Retinas. A Representative forward (top) and orthogonal (bottom) projection micrographs of dye-filled (AL555, cyan) αOFF-S RGCs from Ctrl eyes of WT and WldS+/+ animals. αOFF-S RGCs were identified by modest immunolabeling against SMI-32 (magenta) and dendritic projections within the distal OFF sublamina of the IPL identified by ChAT (red) immunoreactivity. B Mean light-driven spike rate histograms of αOFF-S RGCs from WT and WldS+/+ retinas from Ctrl eyes. C αOFF-S RGC light-evoked mean (p = 0.08), integrated (p = 0.07), and peak (p = 0.03) responses are enhanced by WldS+/+ versus WT Ctrl cells. D RMP is similar for WT and WldS+/+ Ctrl αOFF-S RGCs (p = 0.17). E Sholl analysis of WT and WldS+/+ Ctrl αOFF-S RGC dendrites (p ≥ 0.09). F The average number of dendritic branch points of αOFF-S RGCs is reduced by WldS+/+ (p = 0.005), but dendritic field area (p = 0.52), dendritic length (p = 0.14), and soma area (p = 0.50) are similar to WTs. WT Ctrl group consisted of cells from WT saline (n = 38) and WT naĂ¯ve (n = 10) eyes. WldS+/+ Ctrl group contained cells from WldS+/+ saline-injected (n = 12) and naĂ¯ve (n = 8) eyes. Statistics: Mann-Whitney test (C); Student’s t-test (D, F); Two-Way Repeated Measures ANOVA, Bonferroni post hoc tests (E). Scale bars = 40 μm. Data are expressed as mean ± SEM