Fig. 8

Wld^S+/+ Protects Anterograde Axon Transport and Spatial Acuity Following IOP Elevation in Young Mice. A, B Representative coronal sections (top) of the superior colliculus (SC) following intravitreal injection of CTB-488 (green) into saline- and microbead-injected eyes of WT (A) and Wld^S+/+ (B) mice. Transport deficits indicated by dashed lines. Retinotopic maps (bottom) reconstructed from serial sections of SC with optic disc indicated (white circles). Density of the transported CTB signal ranges from 0% (blue) to 50% (green) to 100% (red). Medial (M) and rostral (R) orientations are indicated. Scale bar = 500 μm. C Quantification of the intact anterograde transport (%) to the SC from saline- and microbead-injected eyes of WT and Wld^S+/+ mice. IOP elevation significantly decreased transport in WT mice (p < 0.001) but transport is preserved in Wld^S+/+ animals (p > 0.99) and significantly greater than WT microbead eyes (p = 0.007). D Difference in spatial acuity threshold (cycles/degree, c/d) between saline and microbead eyes (microbead-control, Δ) from WT and Wld^S+/+ mice. Spatial acuity linearly diminishes over time in WT (R² = 0.80, p = 0.002) but remains stable in Wld^S+/+ mice (R² = 0.18, p = 0.283). Δ spatial acuity is significantly greater in Wld^S+/+ versus WT mice (*, p ≤ 0.05). Statistics: Kruskal-Wallis test, Dunn’s post hoc (C), Linear mixed model (D), Linear regression (D). Data = mean ± SEM