Fig. 2

WGCNA modules demonstrated significant disease-related changes, including enrichment of immunoglobulins in CTE. We generated 28 WGCNA modules from the control, CTE, and FTLD-MAPT proteomic data. A. Representative modules are shown with the top 8 module hub proteins (based on kME value; Table S4) listed below each box plot. Kruskal-Wallis p-values for nonparametric ANOVA following linear modeling of covariates and diagnostic groups are shown in above the box plots along with the number (n) of proteins in each module (module sizes also shown in Table S6). B. (Top): Protein blot of IGHM and IGLL5, key hub proteins in the M28-skyblue module, in control and CTE I-IV as a validation of the proteomic data. GAPDH shown as a loading control. B. (Bottom): Box plots of protein blot densitometry for IGHM and IGLL5. *designates p-value < 0.05 for CTE II cases using ANOVA (IGHM: CTE II significant compared to control, CTE I and CTEIV; IGLL5: CTE II significant compared to control and CTE IV). CTE III cases were borderline significant for both IGHM and IGLL5 in pairwise comparison versus control