Fig. 5

Cell subtype abundance demonstrated enriched glial proteins with a prominent increase of astrocyte proteins in FTLD-MAPT over that of other tauopathies. We utilized proteomic protein level data and the Sharma [24] cell type marker classification in combination with the digital sorting algorithm [26] to determine the aggregate abundance of each cell subtype. The CTE/FTD and AD TMT datasets were kept separate, with p-values (Kruskall-Wallis ANOVA) shown for each cell, however relative abundance was normalized via z-score to allow for comparison across datasets. A. Box plots of astrocyte, microglia and neuron abundances across control, CTE I-IV and FTLD-MAPT as well as control, asymptomatic AD and AD are shown. Protein blot and densitometry was performed for B. GFAP and C. HEPACAM, two astrocyte associated proteins. *designates p-value less than 0.05 (GFAP: FTD-MAPT was significant compared to control and CTE IV; HEPACAM: FTD-MAPT was significant compared to control, AD and CTE IV). As noted in A., astrocyte abundance is greatest in FTLD-MAPT and significantly higher than AD and CTE IV